Many translated example sentences containing “centro organizador” – English- Spanish centrosoma es el único centro organizador de microtubulos [ ]. tema el citoesqueleto. ‘red de filamentos proteicos con función esquelética que constituyen el “andamio” interno de la célula’. se encuentra en todas las. los microtúbulos están anclados por sus extremos negativos al centro organizador de microtúbulos y los extremos positivos se dirigen hacia la periferia ; durante.
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Then the cells were fixed, permeabilized and stained with antibodies as indicated. Furthermore, we should highlight the wide range of cellular activities that HDACs are involved that are appearing in the prior art and have enabled the establishment of new therapeutic approaches to many human pathologies, by using inhibitors and agonists HDACs, and the development of new methods of identification and evaluation of new therapeutic compounds.
Cell Biol 95, ; This definition also includes those compounds or molecules that increase expression of the gene encoding the protein Prganizador. A representative experiment of three is shown. HDAC6 microtubukos co-localized with microtubules acetylated both cell types, concentrating on the site contacting the antigen presenting cell Fig.
M Ribeiro et al.
Arrowheads are directed towards the point of contact of the T cells with antigen presenting cells. The cell conjugates between T cells and antigen presenting dr in the presence of enterotoxigenic superantigen Staphylococcus had a concentration of microtubules acetylated in the contact area with antigen presenting cells Fig.
Thus, an object of the present invention is the use of a mixrotubulos or regulating agent, inhibitor or activator of the activity of HDAC6 protein, hereinafter use of a compound of the present invention, in preparing medicaments or pharmaceutical compositions for the treatment of diseases, disorders or diseases of mammals, preferably human, that develop with alterations of the immune system, preferably with the activation of T cells altered.
However, the staining ogganizador weak in both untreated cells and cells treated with butyrate or trapoxinaB Fig. Histone deacetylase as new cancer drugs. The following examples serve to illustrate the invention and should not be considered as limiting the scope thereof.
DeCS – New terms
In this regard, the gene transfer technology could, by viral vectors, gene be useful in therapy to express tissue-specific manner HDAC6 enzyme in T lymphocytes of patients suffering from such diseases. This definition also includes those compounds that prevent or decrease expression of the gene encoding the HDAC6 protein, ie, preventing or diminuyen gene transcription, the maturation of mRNA, mRNA translation and post-translational modification.
The amount of acetylated microtubules was greater in specific superantigen conjugates that conjugates between cells of the same type or cell conjugates established in the absence of superantigen E Fig. A particular object of the present invention is the method of identifying compounds of the invention in which the determination of the levels of tubulin acetylation ii can be performed by techniques immunoblot or western blot with appropriate antibodies see Example 2 and Figure 2.
Then levels of tubulin and acetylated tubulin were detected by immunoblotting under non-reducing conditions.
Revision sobre filamentos intermedios, con especial referencia a las citoqueratinas.
These data demonstrate that HDAC6 plays a dual role in the reorientation of MTOC regulating the spatial organization of membrane receptors of immune synapse and controlling the reorganization of the microtubule network that occurs during the formation of conjugates between T cells presenting cells antigen.
The immunological synapse and the actin cytoskeleton: Los marcadores de peso molecular se indican a la izquierda. Nature82 ; Grakoui, A.
TCR engagement proline-rich induces tyrosine kinase-2 Pyk2 translocation to the T cell-APC interface Independently of Pyk2 activity and in an immunoreceptor tyrosine-based activation motif-mediated fashion.
Science Viable cells after 24 hours after being transfect das were isolated by centrifugation on a Ficoll gradient and placed in contact with Raji cells to conduct functional studies. Also, from the results of the present invention the importance of acetyl-transferases proteins, which act by increasing levels of tubulin acetylation observed during activation of T cells, and allow it is found: Then the cells were fixed, permeabilized and stained for tubulin.
Microtubupos 4 Figure 4.
Sawing, JM, Nieto, M. Three proteins define a class of human histone-deacetylases related to centdo Hdalp; Proc. Then the cells were stained for LFA-1 and analyzed by confocal microscopy.
Cell conjugates coated coverslips were mounted on Attofluor open chambers Molecular Probes. Trapoxina B fue generosamente cedida por el Dr.
Raji cells were contacted with J77 cells on fibronectin-coated coverslips. The percentage of mature synapse in J77 cells transfected with HDAC6 was two times lower than in cells transfected with the mutant deficient in deacetylation or in lymphocytes treated with TSA and or trapoxin B, or sodium butyrate modified it significantly Fig. In addition, the uncontrolled activation of HDAC6 inhibits translocation of secretory apparatus to the antigen presenting cell and decreases the production of IL-2, which are two events of early and late, respectively activation.