Many translated example sentences containing “centro organizador” – English- Spanish centrosoma es el único centro organizador de microtubulos [ ]. tema el citoesqueleto. ‘red de filamentos proteicos con función esquelética que constituyen el “andamio” interno de la célula’. se encuentra en todas las. los microtúbulos están anclados por sus extremos negativos al centro organizador de microtúbulos y los extremos positivos se dirigen hacia la periferia ; durante.
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Asterisks mark the antigen presenting cells. And, more specifically to the field of methods of identifying novel therapeutic compounds and pharmaceutical compositions for treating diseases or disorders splenic immune system disorders. From this moment, tubulin acetylation increased progressively reaching a peak after two hours of stimulation Fig. El fragmento de fibronectina de 80 kD FN80 fue generosamente cedido por la Dr. FTY fingolimod in multiple sclerosis: Therefore, the regulation of desacetiladora activity tubulin mediated HDAC6 and its implications for modulating the activation of T cells, disclosed herein for the first time, it can be an effective treatment for diseases associated with an immune response and also exacerbated in those that occur with a deficient immune response.
Nature; Furumai, R. These data demonstrate that HDAC6 plays a dual role in the reorientation of MTOC regulating the spatial organization of membrane receptors of immune synapse and controlling the reorganization of the microtubule network that occurs during the formation of conjugates between T cells presenting cells antigen.
By contrast, acetylation levels not varied in the presence of superantigen B Fig. Thus, an object of the present invention is the use of a compound or regulating agent, inhibitor or activator of the activity of HDAC6 protein, hereinafter use of a compound of the present invention, in preparing medicaments or pharmaceutical xentro for the treatment organuzador diseases, disorders or diseases of mammals, preferably human, that develop with alterations of the immune system, preferably with the activation of T cells altered.
Method of identifying compounds according to claim 1 wherein determining a parameter related to the activation of T cells ii is carried outamong other possible, by a technique belonging to the following group: The time elapsed since the initial contact time is indicated in minutes in the upper left corner of each image.
Inactivation of glycogen synthase kinase 3 promotes axonal growth and recovery in the CNS. These data clearly showed that could distinguish two phases during tubulin acetylation mediated superantigen.
Cell Biol 95, ; Figura 2 Figure 2. Actin microfilaments are considered the most important components for activation of T cells Valitutti, SM, Dessing, M. Cell Biol, 98, ; Exclusion of CD43 from the immunological synapse is mediated by phosphorylation-regulated relocation of the cytoskeletal adaptor moesin. HDAC6 partially co-localized with microtubules acetylated both cell types, concentrating on the site contacting the antigen presenting cell Fig. Then the cells were fixed, permeabilized and stained for tubulin.
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Exp Med; En cada experimento se contaron al menos conjugados celulares. A particular object of the present invention is a method for identifying potential inhibitors of the invention where the point T i lymphocytes can be stimulated or prepared by methods known in the prior art compounds see Example ; stimulation antigen presenting cells Raji cells more SEE and transformation of cell cultures of T cells with a genetic construct allows expression of HDAC6 gene allow better assessment of the effect of such potential inhibitor compounds.
Kind code of ref document: This definition also includes those compounds that prevent or decrease expression of the gene encoding the HDAC6 protein, ie, preventing or diminuyen gene transcription, the maturation of mRNA, mRNA translation and post-translational modification. Three proteins define a class of human histone-deacetylases related to yeast Hdalp; Proc. Method for identifying potential inhibitors of claim 1 wherein the T point i lymphocytes can be stimulated or prepared by methods, among others, belonging to the following group: Trapoxin B was generously provided fe Dr.
Confocal sections distanced 0. Compounds inhibitors or antagonists of HDAC6, which consequently produce a net tubulin acetylation, are useful in restoring impaired immune response.
The object of the present invention is a method of identifying and evaluating the activity regulating compounds HDAC6 protein on T cell activation, comprising the following steps: Nature; Matsuyama, A. The membrane-microfilament linker ezrin is involved in the organizaor of the immunological synapse and in T cell activation. Method of identifying compounds regulators tubulin deacetylase activity of HDAC6, and applications.