2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.

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Incubate the cores for 1. Examine the cores as you weigh them. This increases the tissue weight and may lead to small errors. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel. Repeat for all solutions. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel.

Cell Transport The movement of molecules can be either passive no energy or active needs energy depending upon chardakoov membrane structure and concentration. Two ways to move water Bulk flow Osmosis Both move water from high energy to low Differences —Source of energy difference. Boy, Oh Buoyancy Does chardakvo Float? Does it correspond to the value obtained by the Chardakov technique?


Slowly release a drop of the methylene blue solution from the pipette and note whether the drop of the dye sinks, disperses, or floats to the surface in this solution and subjectively estimate whether it does so rapidly or slowly.

Complete Tables 1 and 2. My presentations Profile Feedback Log out. Divide by 10 6 to convert to MPa. Weigh the cores to the nearest 0.

Be sure not to include any fragments of the skin. Chardakov Method for Determining Water Potential. No change in chaddakov, concentration No net water movement Tissue and solution were at the same WP If we can find this solution concentration —We can find the tissue WP.

Does a gas differ from other fluids? Then remove the tissues, gently blot on paper towels and reweigh. This is due to incipient plasmolysis that occurs at low solution water potential.

Plant Physiology

Use the following equation to calculate the percent change in weight for each tissue by the following equation: Dispense 10 chaedakov of water or a sucrose solution 0. Share buttons are a little bit lower. The Chardokov method provides a quick means to determine plant tissue water potentials.

Alternately, for a more accurate measurement of changes in the solution density, a refractometer can be used.

Auth with social network: Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to dye the sucrose solution, dip a dry probe into methylene blue powder and chardzkov mix. Hypertonic Concentration with higher solute concentration and less water concentration Hypotonic lower solute concentration and more water concentration. Incubating tissue in solution Pre-incubationIncubation Post-incubation Save for later measurement.


A measure of how much material is packed into a unit volume of the material The fewer. Do the cores show various degrees of turgor? Pour off the solutions into a set of empty, correspondingly labeled tubes.

Then, the percent change in weight of the tissue is plotted versus solution concentration or osmotic potential. We think you have mfthod this presentation.

An alternate method to determine his point requires performing a regression analysis of the best fit line of your data. About project SlidePlayer Terms of Service. Be sure to use a different pipet for each dye stock.

Chardakov method

Put two or preferably three potato cores in each solution water or sucrose. Which method do you think will be more accurate? Use a cork borer to prepare at least 27 uniform tissue vhardakov from the potato.